FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_lb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_lb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24061586
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAG684700.013.761559570
TATACTG149300.013.596495
TAGACCG34450.013.1056665
AATAGAG591350.012.93893270
CTATACT84450.012.8890574
TATACCG46900.012.7609185
TATACAG193150.012.6479265
CCCTTAG41100.012.6042861
GTATAGA119400.012.1658411
TTATACT148500.012.1142654
CTACACT92250.011.988954
GAATAGA635600.011.98857270
GTATTAG86500.011.9777141
CTTAGAC65200.011.9707223
GTGTAGG93150.011.9117281
TCTATAC112800.011.5734493
GTATAGG63500.011.5204891
ACTGTGC105250.011.4723398
TAATACT132000.011.37484
GTGTTAG115050.011.2871841
Produced by FastQC (version 0.11.2)