FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_lb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_lb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22642914
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250600.1106748009553894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAG749450.014.43155170
AATAGAG596550.014.16405470
TAGACCG30250.013.8838495
CCCTTAG39450.013.2208541
CTTAGAC61900.013.0611413
GAATAGA675200.012.71634570
GTATAGG58500.012.3861031
CTATACT79150.012.2486354
GTATTAG81000.012.1002191
TATACTG147000.012.0948485
ACTGGTC75900.012.0814048
TATACAG182700.012.049425
TATACCG44900.011.9261195
GTATATA289100.011.7326441
TAGAGAG375250.011.71564470
ACGAATA940600.011.61431769
GTGTAGG80600.011.5956861
TCTATAC106650.011.5844023
GTATAGA114650.011.4797921
TTATACT138750.011.4772344
Produced by FastQC (version 0.11.2)