FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_bay2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_bay2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24658202
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG48950.013.5153781
TAGACCG33300.013.4530145
CGAATAG521350.013.29303370
TATACTG168500.012.8571485
TATACAG213400.012.7596595
CTTAGAC70950.012.5791523
CTATACT86900.012.44508654
CCTATAC63900.012.3238173
GTATTAG95550.011.979431
GTATAGG68150.011.9676571
TTATACT168450.011.6352634
TACACCG43300.011.23525
GTATTAT214100.011.1339571
CTACACT94900.011.1009324
GTATATA374000.010.9692041
GTATAGA139300.010.9058041
TAATACT153450.010.788314
GGTGTAG157500.010.71189268
ACAGTGC109250.010.6999678
TACACTG167700.010.68545
Produced by FastQC (version 0.11.2)