FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_bay1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_bay1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003357
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG30100.015.1159475
CCCTTAG43500.014.8867591
CTATACT76150.014.5236464
CTTAGAC66850.013.6648083
CGAATAG383250.013.29732670
TATACTG142550.013.1354525
TATACAG186100.012.9578025
CCTTAGA60450.012.2167442
GTATTAG83200.012.1588421
CCTATAC54400.012.031153
GTATAGG60900.011.8979121
TAATACT133350.011.7845184
GTGTAGG84950.011.6611331
CTACACT86650.011.632754
GTGTTAG102750.011.61691
GTATAGA121050.011.4222081
ACAGTGC96000.011.3746458
TATACCG44050.011.3618385
TTATACT144250.011.3307734
TACACAG142750.011.180145
Produced by FastQC (version 0.11.2)