FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_lb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_lb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24061586
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG575860.2393275322748883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG34400.014.9573575
ATCTCGT109800.014.91762970
ACAGGCA506150.014.54173870
AATCTCG109850.014.46478869
TATACTG150000.013.5808755
CTTAGAC66450.013.3265283
TATACCG48650.013.3102095
GACAGGC578950.013.14240370
CCCTTAG44750.013.0663661
AGACAGG749950.012.97851570
TATACAG193150.012.8664765
GTATAGG63400.012.8123891
GTATATA269500.012.71906951
GTATAGA112150.012.3943181
CTATACT86050.012.3249474
GTGTTAG113700.012.2561471
CTACACT88700.012.2329554
TACACCG44450.012.205515
TACACTG164650.012.0110985
ACAGTGC103550.011.8636378
Produced by FastQC (version 0.11.2)