FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_lb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_lb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22642914
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG583250.257586103979373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC473050.017.0535970
GATCTCG98250.016.31495369
ATCTCGT103850.015.50255570
CGTACTA273800.014.968470
CCGTACT350450.014.81040870
GACCGTA587850.014.70558670
AGACCGT751150.014.467278570
TAGACCG27700.013.7738165
GTATAGG60450.013.206481
CCCTTAG37100.012.9299021
GTATAAG91500.012.6664671
TATACAG178200.012.649865
GTATAGA107200.012.6078521
GTATATA265750.012.5696651
GAGACCG908900.012.05646269
TATACTG152450.012.0542295
CTTAGAC60300.012.01577953
TTATACT139050.012.0074064
GTGTAGG81850.011.9353211
AGATCTC138750.011.88066868
Produced by FastQC (version 0.11.2)