FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_bay2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_bay2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24658202
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG689470.2796108167172935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG35700.016.5696245
CCCTTAG49550.014.2031851
TATACTG173500.014.1218875
CATCTCG96650.013.724380569
ATCTCGT101950.013.07953570
AGACTCC545100.012.96327470
TATACAG216550.012.94697955
GTATAGG68150.012.9469661
ACTCCTG415950.012.81484370
CTATACT93100.012.5570184
GTCCTAG31600.012.5205881
CTTAGAC76050.012.47263053
TCCTGAG240850.012.35170370
GTATATA341500.012.3443751
TAATACT161100.012.2321444
TTATACT173100.012.0918824
ACTGTGC106450.012.0666658
GTATTAG94700.011.905271
TACACTG173450.011.8456255
GACTCCT444200.011.75560170
Produced by FastQC (version 0.11.2)