FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_bay1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_bay1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003357
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG597510.2715540178710003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG43950.015.8542461
ACGGACT269050.014.88144370
TAGACCG29650.013.930215
GTATAGG56100.013.8561171
TATACTG149800.013.8328045
CTATACT79600.013.8074974
CTTAGAC66450.013.3794453
TATACAG186800.012.6668955
GTATATA285100.012.5763361
AGACGGA414600.012.49350870
GTATAGA109600.012.4276911
TTATACT149250.012.3827754
GTATAAG92500.012.3403441
TCTATAC106500.012.16050153
GTATTAT176800.012.12050151
ACTGTGC96000.012.1039398
GACGGAC341450.011.98230669
GTATTAG83900.011.9776421
GTGTTAG100200.011.9162071
CCTTAGA59850.011.696832
Produced by FastQC (version 0.11.2)