FastQCFastQC Report
Fri 26 Jan 2024
HVF32AFX5_n01_DAM-GR_bZIP1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF32AFX5_n01_DAM-GR_bZIP1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24348994
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGGGGTT492480.20225886950401317TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT471950.1938273096621569TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGCGGAT461820.18966697351028136TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCGCGTAT443790.18226215013236274TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGCGTAT388240.15944806590366734TruSeq Adapter, Index 4 (97% over 38bp)
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA377760.1551439866468405No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCGCGGAT369130.15159969237332763TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGGGGGT363130.1491355248598772TruSeq Adapter, Index 4 (97% over 38bp)
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA313080.12858026085184462No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG312260.12824349129167306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGCGGTT304350.12499489711977423TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGGGGAT293150.12039511776133338TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGTCGTAT258060.10598384475350399TruSeq Adapter, Index 4 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)