FastQCFastQC Report
Fri 26 Jan 2024
HVF32AFX5_n01_DAM-GR-GBF2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF32AFX5_n01_DAM-GR-GBF2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10340580
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTAT8518218.237652046596999TruSeq Adapter, Index 8 (97% over 36bp)
CTATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGG696830.6738790280622557Clontech Universal Primer Mix Short (100% over 22bp)
ATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGGAA672930.6507662046036102Clontech Universal Primer Mix Short (100% over 22bp)
TATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGGA651390.6299356515785381Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAGAGATCGG550610.5324749675550114Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAAGATCGGA493060.4768204491430848Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAGATCGGAA451050.43619410129799296Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCGCGTAT408220.39477476118360866TruSeq Adapter, Index 8 (97% over 36bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAAGATCGGA388850.3760427364809324Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAGAGATCGG362820.35087006724961267Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAGATCGGAA265400.2566587174026989Clontech Universal Primer Mix Short (100% over 22bp)
TAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGGAAG159950.15468184569917742Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATATCGTAT157380.15219649187956574TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGGAT151840.1468389587431266TruSeq Adapter, Index 8 (97% over 36bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTATAGGG132630.12826166423933666Clontech Universal Primer Mix Long (95% over 23bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCAC126020.12186937289784519Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGAGATCGGAAGAGCAC123930.11984820967489251Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGCTCTCGTAT119890.1159412721530127TruSeq Adapter, Index 8 (97% over 36bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTAAGATCGGAAG113940.11018724288192731Clontech Universal Primer Mix Long (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)