FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32263813
Sequences flagged as poor quality0
Sequence length110
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18656915.782611621261256No Hit
TGCCGCTATTTCTCTGGGTACCTTAGCACCGTTCCTCGCTATTATTCCAA5352211.658889480917832No Hit
TGCCGCTATTTCTCTGGGTACCCGCAACAGTTTCTCGCTATTATTCCAAC4724751.464411537470788No Hit
TGCCGCTATTTCTCTGGGTACCGGACACCATTTCTCGCTATTATTCCAAC4098661.2703582183544146No Hit
TGCCGCTATTTCTCTGGGTACCTGAGATAAGTTCTCGCTATTATTCCAAC3335941.0339571457347585No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT758560.2351117023892991No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG680090.21079033652965942No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC551540.1709469367430316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG763600.082.945082
ATGGTTA1857650.079.67925104
AAGCAGT830400.076.684711
ACGATCG121950.074.278641
CCGCTAT3109500.072.4816063
TCCACAA484250.071.14784104
GTATCAA897500.070.7791759
TTTCTCT3233300.070.455789
CTATTTC3236500.070.06356
GCCGCTA3263900.069.648592
GCTATTT3359400.068.1182865
GGTATCA950800.066.9563758
GCAGTGG962600.066.0656053
TGCCGCT3430400.065.976771
AGTGGTA985000.064.7466055
GTGGTAT989600.064.3941046
CAGTGGT992300.064.098284
CCGCTAG40650.059.8610883
TATTTCT3923700.058.33417
GCCGCGA56800.057.334182
Produced by FastQC (version 0.11.5)