FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf2_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf2_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21045354
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG570290.2709814242136293No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA525030.2494754899347381No Hit
CCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTAT436050.20719537433297627No Hit
CAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG420360.199740047138195No Hit
CCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGA395320.18784193413900283No Hit
CCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTG392960.18672054649211414No Hit
CATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT385230.18304752678429642No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT380440.18077148999251805No Hit
GCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAG358230.17021809184107808No Hit
TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTA357180.16971916937106404No Hit
TCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGT350370.16648330077983006No Hit
GTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCC336300.15979773968164185No Hit
CGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGAT334970.15916577121962405No Hit
ATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCT333070.15826295913102723No Hit
TGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTA332970.1582154427053116No Hit
TTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCT323990.1539484676760486No Hit
GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATAT318420.15130180276368838No Hit
TTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGAC316590.15043225217309245No Hit
GGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTT311800.1481562153813141No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG310750.14765729291130006No Hit
TCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGT310370.14747673049358068No Hit
ATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCA309650.14713461222842816No Hit
CTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGA307350.14604173443696886No Hit
CAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTC289170.1374032482418685No Hit
TAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT287990.13684255441842413No Hit
CCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGC281000.13352115626090205No Hit
GATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTA280580.13332158727289645No Hit
TTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGAT269580.12809478044417785No Hit
ATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAA253460.12043513261881934No Hit
GGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTAC249780.11868652815248439No Hit
CATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGC243250.11558370555325417No Hit
ACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTG239880.11398240200663767No Hit
ATTTAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTAC237160.1126899552271727No Hit
GTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTT235420.11186316941972085No Hit
ATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTC231810.11014782645138683No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG228860.10874609189277595No Hit
TATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCA228670.10865581068391628No Hit
CTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGC225760.10727308269559163No Hit
CTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGC223290.10609942698041573No Hit
GTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATA221170.1050920787552445No Hit
AAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTT214880.10210329557773178No Hit
AACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCT213340.10137154262171118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT889350.088.806011
AGCAGTG947200.083.036152
GGTATCA1070500.073.566688
TGGTATC1075850.073.144217
CAGTGGT1074150.073.116634
GTGGTAT1082150.072.6843956
GCAGTGG1092100.071.8461763
AGTGGTA1099300.071.564485
GTATCAA1115500.070.722679
AGAGTAC1079050.039.7130118-19
GCAGAGT1081250.039.7056716-17
CAACGCA1096050.039.31646312-13
ATCAACG1114200.038.7385110-11
TACATGG1121700.037.82420722-23
CAGAGTA1084450.036.2784618-19
CATGGGG364800.036.0172124-25
AACGCAG1104700.035.7493514-15
TCAACGC1110700.035.55087712-13
AGTACAT1205050.035.4771820-21
ACGCAGA1236600.034.8800214-15
Produced by FastQC (version 0.11.5)