FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf2_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf2_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31152914
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG846750.2718044289532594No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA781880.2509813367699728No Hit
CCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTAT643980.20671581477097134No Hit
CAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG622200.1997244944726519No Hit
CCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGA590410.18951999161298363No Hit
CCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTG586100.18813649342722805No Hit
CATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT571060.1833086946537329No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT565960.18167160863346524No Hit
GCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAG533940.17139327640425547No Hit
TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTA526940.16914629559212344No Hit
TCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGT520840.16718821231297978No Hit
GTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCC497740.15977317563294402No Hit
ATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCT491070.157632123916241No Hit
TGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTA489360.15708321860356306No Hit
CGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGAT486720.1562357858401304No Hit
TTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCT485660.15589552874572182No Hit
TTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGAC469020.15055413435802506No Hit
GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATAT467970.15021708723620525No Hit
TCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGT461330.14808566543726856No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG460900.1479476366159519No Hit
GGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTT456190.14643573952664588No Hit
CTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGA455770.14630092067791797No Hit
ATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCA455140.14609869240482606No Hit
CAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTC432000.13867081583443525No Hit
TAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT423040.13579468039490622No Hit
GATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTA415690.13343535054216757No Hit
CCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGC408830.13123330934627817No Hit
TTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGAT393730.12638625073725046No Hit
ATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAA377830.12128239432112195No Hit
GGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTAC371220.11916060243995152No Hit
CATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGC357900.1148849189517231No Hit
ACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTG354080.11365870942281675No Hit
ATTTAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTAC350850.11262188827664725No Hit
GTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTT349070.11205051315584795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG345000.11074405431222262No Hit
ATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTC343310.11020156894472216No Hit
TATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCA335500.10769458035290054No Hit
CTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGC334100.10724518419047413No Hit
CTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGC330430.10606712425039917No Hit
GTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATA327460.10511376239153744No Hit
TACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACA318030.10208675824033668No Hit
AACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCT315870.1013934041611645No Hit
AAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTT312270.10023781402921088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1321950.088.5437241
AGCAGTG1411100.082.798632
GGTATCA1579600.073.8933648
CAGTGGT1583650.073.5971764
TGGTATC1587600.073.512587
GTGGTAT1592450.073.272836
GCAGTGG1616450.072.169833
AGTGGTA1628700.071.6695
GTATCAA1639400.071.223389
GCAGAGT1601450.039.89867816-17
AGAGTAC1598600.039.8830918-19
CAACGCA1623350.039.46230712-13
ATCAACG1651550.038.85862410-11
TACATGG1667000.037.70551322-23
CAGAGTA1604950.036.38636418-19
CATGGGG539950.036.3153524-25
AACGCAG1631550.035.90329414-15
TCAACGC1640450.035.73819412-13
AGTACAT1776550.035.7103520-21
ACGCAGA1824100.035.1496714-15
Produced by FastQC (version 0.11.5)