FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf2_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf2_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26293911
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG711320.2705265108716615No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA656530.24968898692933125No Hit
CCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTAT548200.20848933427971214No Hit
CAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG518880.19733846364658345No Hit
CCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGA498800.18970171459088No Hit
CCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTG491280.18684173685687153No Hit
CATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT485650.18470055671824553No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT477000.18141082169175973No Hit
GCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAG450130.171191725719312No Hit
TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTA445460.16941564912119766No Hit
TCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGT443140.168533315564961No Hit
ATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCT420330.1598583033159274No Hit
GTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCC417790.15889230019832348No Hit
CGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGAT415490.1580175729658475No Hit
TGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTA411910.1566560410126892No Hit
TTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCT406840.1547278379393617No Hit
GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATAT396660.15085621914518538No Hit
TTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGAC395170.15028954802501615No Hit
TCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGT388300.14767677581322913No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG386500.14699220667476967No Hit
CTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGA382720.14555461148400478No Hit
ATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCA382020.14528839015238165No Hit
GGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTT380360.14465706528024683No Hit
CAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTC363390.13820309956932614No Hit
TAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT353670.13450642622164502No Hit
GATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTA347370.13211043423703686No Hit
CCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGC342510.1302620975631963No Hit
TTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGAT333790.12694574040354817No Hit
ATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAA319030.12133227346818051No Hit
GGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTAC309010.11752150526408947No Hit
CATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGC307070.11678369185930536No Hit
ACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTG299430.11387807618273296No Hit
ATTTAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTAC294640.1120563616420547No Hit
ATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTC292260.11115120911453606No Hit
GTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTT291190.11074427079334072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286500.10896058787146574No Hit
TATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCA283960.10799458475386184No Hit
CTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGC281130.10691828994172833No Hit
CTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGC280550.10669770655266916No Hit
GTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATA276100.10500529951592213No Hit
AACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCT268480.10210729016311038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1112000.088.3591
AGCAGTG1187050.082.678092
GGTATCA1329650.073.69048
CAGTGGT1336650.073.279194
TGGTATC1337400.073.183677
GTGGTAT1341850.073.023186
GCAGTGG1369950.071.470443
AGTGGTA1373900.071.386765
GTATCAA1379500.071.090929
AGAGTAC1344350.039.9126318-19
GCAGAGT1350800.039.83063516-17
CAACGCA1362950.039.6020812-13
ATCAACG1387250.038.9675510-11
TACATGG1393900.037.96912422-23
CATGGGG449050.036.410724-25
CAGAGTA1351000.036.30576318-19
AACGCAG1375450.035.8340914-15
AGTACAT1494500.035.77975520-21
TCAACGC1376250.035.77884312-13
ACGCAGA1549150.034.90629614-15
Produced by FastQC (version 0.11.5)