FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf2_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf2_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26186551
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG704210.2689204851757683No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA652640.2492271700843689No Hit
CCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTAT545070.2081488318182872No Hit
CAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG518740.19809405217204815No Hit
CCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGA491870.187833059802339No Hit
CCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTG490540.18732516550194028No Hit
CATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT483090.18448019366887988No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT474120.18105477120679236No Hit
GCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAG445200.1700109342387243No Hit
TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTA441840.16872783284824336No Hit
TCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGT436430.16666188685940353No Hit
GTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCC414330.15822244021368068No Hit
ATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCT413590.1579398524074438No Hit
TGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTA410890.15690878879009307No Hit
CGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGAT410780.15686678249457134No Hit
TTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCT406580.15526290575647017No Hit
GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATAT392550.1499051937003846No Hit
TTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGAC392370.14983645612589455No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG388810.1484769796526469No Hit
TCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGT387870.14811801676364328No Hit
ATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCA382740.14615899589067685No Hit
GGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTT381180.14556327024509644No Hit
CTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGA380300.14522721988092285No Hit
CAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTC361690.1381205184294793No Hit
TAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT349210.13335471326483583No Hit
GATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTA344880.1317011927229363No Hit
CCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGC342120.1306472165807555No Hit
TTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGAT332180.1268513749672494No Hit
ATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAA318590.12166168809325062No Hit
CATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGC308510.11781238392180779No Hit
GGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTAC305690.11673549525479703No Hit
ACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTG297650.11366521692757477No Hit
ATTTAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTAC291740.1114083332318181No Hit
GTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTT290470.11092335145624942No Hit
ATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTC288550.1101901506616889No Hit
TATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCA282250.10778433555453713No Hit
CTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGC281450.10747883522347025No Hit
CTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGC280760.10721534118792506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275570.10523340779012859No Hit
GTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATA274800.10493936372147673No Hit
AACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCT267200.10203711057634127No Hit
TACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACA264840.10113588459969393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1098550.088.6015851
AGCAGTG1174650.082.744852
GGTATCA1318350.073.6264958
CAGTGGT1325300.073.270244
TGGTATC1328200.073.102577
GTGGTAT1334050.072.8470156
GCAGTGG1345700.072.1037753
AGTGGTA1363050.071.323035
GTATCAA1368550.070.932039
GCAGAGT1335100.039.81603216-17
AGAGTAC1332250.039.7869918-19
CAACGCA1350950.039.4439212-13
ATCAACG1377250.038.7553710-11
TACATGG1380900.037.80495522-23
CAGAGTA1339600.036.2321518-19
CATGGGG451200.035.8109324-25
TCAACGC1364050.035.6471812-13
AACGCAG1366750.035.61127514-15
AGTACAT1484550.035.55216220-21
ACGCAGA1528850.034.90033714-15
Produced by FastQC (version 0.11.5)