FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf2SBC_202009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf2SBC_202009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28895933
Sequences flagged as poor quality0
Sequence length110
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGCTATTTCTCTGGGTACCCGCAACAGTTTCTCGCTATTATTCCAAC755094726.13152169199728No Hit
TGCCGCTATTTCTCTGGGTACCTTAGCACCGTTCCTCGCTATTATTCCAA672549723.2748913142898No Hit
TGCCGCTATTTCTCTGGGTACCGGACACCATTTCTCGCTATTATTCCAAC554364019.184845147585303No Hit
TGCCGCTATTTCTCTGGGTACCTGAGATAAGTTCTCGCTATTATTCCAAC463831916.05180562953271No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2121060.7340340940020867No Hit
TGCCGCTATTTCTCTGGGTACCACATGAGCATTCTCGCTATTATCCAACC1613050.5582273463881577No Hit
TGCCGCTATTTCTCTGGGTACCAGCCTCTCATTCTCGCTATTATTCCAAC1439480.49816006979252064No Hit
TGCCGCTATTTCTCTGGGTACCATCACCTGGTTCTCGCTATTATTCCAAC1144300.39600728586960665No Hit
TGCCGCTATTTCTCTGGGTACCAGTGGTCCTTTCTCGCTATTATTCCAAC1014980.3512535829869207No Hit
TGCCGCTATTTCTCTGGGTACCTTAGCACCGTTCCTCGCTATTATTCAAA388740.13453104282876072No Hit
TGCCGCTATTTCTCTGGGTACCGGACACCATTTCTCGCTATTATTACAAC351840.12176107966474037No Hit
TGCCGCTATTTCTCTGGGTACCTTAGCACCGTTCCTCGCTATTATTACAA294120.10178595029272804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTCG306.5774657E-9103.9950267
CTAGTGC450.0103.994846
TCCCACT306.5792847E-9103.991791
TGCCGCT27464550.0103.857561
GCCGCTA27516600.0103.791682
GCCACTA22350.0103.751772
CCGCTAT27579750.0103.5597843
GACGCTA98850.0103.511042
CTATTTC27659650.0103.4576
TATTTCT27702400.0103.370177
GCTATTT27733900.0103.3595
CTATGTC12350.0103.152786
TTTCTCT27628200.0103.134049
CCGCTAG29750.0103.112193
GCCGATA45500.0103.070262
CCGCGAT54450.0103.031143
GCCGGCT5050.0102.954852
TTCCGCT26700.0102.823331
TGCCACT21750.0102.796471
GCCCCTA12900.0102.775282
Produced by FastQC (version 0.11.5)