FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf1_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf1_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24682029
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG626130.25367849620466776No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA612930.24833047558610355No Hit
CCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTAT506080.20503986929113488No Hit
CATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT479300.19418986988468412No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT461990.18717667011897604No Hit
CCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGA460600.18661350734171814No Hit
CCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTG454760.18424741337108064No Hit
GCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAG415200.16821955763847454No Hit
CAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG415180.16821145457693126No Hit
ATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCT413190.16740519995337497No Hit
TGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTA397580.16108076041884564No Hit
TCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGT385770.1562959025775393No Hit
TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTA383880.15553016326169944No Hit
CGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGAT377660.15301011112173962No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG374700.15181085801333433No Hit
GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATAT361620.14651145576402977No Hit
CAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTC359970.14584295318670926No Hit
TCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGT353630.14327428267748976No Hit
GTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCC349110.14144299076870867No Hit
ATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCA342820.13889457791334742No Hit
TTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGAC339330.1374805936740452No Hit
TTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCT335270.13583567218075954No Hit
GATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTA331230.13419885374901713No Hit
CTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGA324860.13161802864748276No Hit
GGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTT324330.13140329751658586No Hit
CCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGC322470.1306497127930609No Hit
CATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGC298830.12107189404890496No Hit
ACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTG296850.12026969095612033No Hit
ATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTC289940.11747008319291741No Hit
ATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAA287440.11645720050000752No Hit
TTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGAT286610.11612092344596144No Hit
TAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT275670.11168854878178774No Hit
CTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGC271490.10999500891924242No Hit
AACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCT269630.10924142419571747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261880.1061014878476968No Hit
GTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATA257860.10447277247749769No Hit
GGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTAC256580.10395417653872782No Hit
GTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTT251600.10193651421445134No Hit
ATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTGGGTAC249970.10127611469867408No Hit
TATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCA248150.10053873609823569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1490100.093.153311
AGCAGTG1612400.085.914242
TGGTATC1840650.075.1434567
GCAGTGG1844850.074.900343
CAGTGGT1849000.074.849194
GTGGTAT1867300.074.1273046
GGTATCA1869250.074.077798
AGTGGTA1876350.073.841075
GTATCAA1919550.072.172969
CAACGCA1886000.040.34821312-13
AGAGTAC1881250.040.29203418-19
GCAGAGT1886300.040.2791616-17
ATCAACG1929200.039.53290610-11
TACATGG1929200.038.62183422-23
CATGGGG601050.037.61600524-25
ACGCAGA2027950.037.57058714-15
AGTACAT2021400.037.32810220-21
TCAACGC1892000.036.66082412-13
CAGAGTA1895900.036.5479418-19
AACGCAG1915500.036.25086614-15
Produced by FastQC (version 0.11.5)