FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf1_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf1_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31494530
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG797300.25315507169022683No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA783280.24870350502134816No Hit
CCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTAT652690.20723916184810504No Hit
CATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT614240.19503069263138711No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT595610.18911537971831935No Hit
CCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGA583520.18527661787618357No Hit
CCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTG578440.18366363936848715No Hit
CAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG528000.16764815985506054No Hit
ATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCT526530.16718141213728227No Hit
GCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAG521670.16563828702952543No Hit
TGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTA509360.16172967178745007No Hit
TCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGT492200.15628110659216063No Hit
CGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGAT489200.15532856022934777No Hit
TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTA488310.15504597147504662No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG482400.1531694551403053No Hit
GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATAT461610.1465683088460123No Hit
CAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTC461580.14655878338238418No Hit
TCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGT447970.14223739804975657No Hit
GTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCC441650.14023070037876417No Hit
ATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCA435590.13830655672588224No Hit
TTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGAC432730.13739846252666732No Hit
GATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTA428210.13596329267336263No Hit
TTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCT422920.13428363592026932No Hit
GGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTT416960.13239124381281447No Hit
CCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGC412940.13111483168664526No Hit
CTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGA411410.13062903304161072No Hit
ACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTG379120.12037645902320182No Hit
CATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGC378250.12010022057798608No Hit
ATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTC370730.11771250436186854No Hit
ATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAA365040.11590584142706686No Hit
TTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGAT356660.11324506192027632No Hit
TAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT348160.11054618055897326No Hit
CTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGC347390.11030169365918463No Hit
AACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCT340300.1080505090884036No Hit
GTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATA329720.10469119558221697No Hit
GGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTAC325370.10331000335613837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG324920.10316712140171642No Hit
ATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTGGGTAC323610.1027511761566215No Hit
TATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCA317370.10076987972197075No Hit
GTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTT316530.10050316674038316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1884850.092.8355561
AGCAGTG2029950.085.9758762
TGGTATC2320350.075.206937
GCAGTGG2326350.074.851043
CAGTGGT2330850.074.765954
GTGGTAT2352200.074.203966
GGTATCA2354850.074.1691748
AGTGGTA2374500.073.549695
GTATCAA2413250.072.493879
CAACGCA2380300.040.41359712-13
AGAGTAC2372350.040.40699818-19
GCAGAGT2376300.040.38269816-17
ATCAACG2426450.039.70514710-11
TACATGG2429350.038.79298822-23
ACGCAGA2559350.037.62421414-15
CATGGGG757750.037.47165324-25
AGTACAT2555850.037.30500820-21
TCAACGC2381650.036.73766712-13
CAGAGTA2393250.036.57531718-19
AACGCAG2416100.036.2808814-15
Produced by FastQC (version 0.11.5)