FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf1_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf1_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26714018
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA659130.2467356277142585No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG655050.24520833968143615No Hit
CCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTAT545820.20431969462624452No Hit
CATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT519240.19436986229476974No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT496520.18586496422964152No Hit
CCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGA492930.18452110049487874No Hit
CCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTG479430.17946757391568727No Hit
ATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCT438140.16401126928940454No Hit
CAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG435300.1629481570312635No Hit
GCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAG432600.16193745171542523No Hit
TGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTA424930.1590662999478401No Hit
TCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGT408420.15288602410914No Hit
CGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGAT407960.1527138298701453No Hit
TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTA405320.15172558467243677No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG399530.14955818327291687No Hit
CAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTC386540.14469556769782815No Hit
GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATAT379050.14189179628463228No Hit
TCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGT376950.1411056921500914No Hit
ATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCA364390.13640404075493248No Hit
GTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCC361740.1354120522042023No Hit
TTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGAC359320.13450616077296945No Hit
GATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTA356800.1335628358115204No Hit
TTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCT348480.13044836609752977No Hit
CCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGC337930.12649912865971716No Hit
CTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGA337570.12636436795093872No Hit
GGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTT336270.1258777320581277No Hit
ACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTG320570.12000066781417905No Hit
CATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGC316810.11859316707804868No Hit
ATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTC311110.11645945585572338No Hit
ATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAA302700.11331129596453818No Hit
TTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGAT295600.1106535153191856No Hit
CTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGC291760.10921606775888225No Hit
AACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCT288640.10804814161613577No Hit
TAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT284260.10640855299266475No Hit
GTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATA279220.1045219030697666No Hit
ATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTGGGTAC269310.10081224022533788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268080.1003518078036782No Hit
GGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTAC267890.10028068409626736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1595200.092.655281
AGCAGTG1711850.086.028312
TGGTATC1952000.075.3914957
GCAGTGG1948250.075.274763
CAGTGGT1957150.075.0729754
GGTATCA1985300.074.218068
GTGGTAT1982350.074.214056
AGTGGTA1996250.073.768715
GTATCAA2038050.072.442839
CAACGCA2002550.040.5099712-13
AGAGTAC1995900.040.44954318-19
GCAGAGT2000500.040.35862716-17
ATCAACG2051700.039.63498710-11
TACATGG2044650.038.86342222-23
ACGCAGA2162400.037.51692614-15
CATGGGG649250.037.4585124-25
AGTACAT2153850.037.3211320-21
TCAACGC2005950.036.88702412-13
CAGAGTA2008150.036.77681718-19
AACGCAG2039800.036.31220214-15
Produced by FastQC (version 0.11.5)