FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf1_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf1_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25463030
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG649660.25513852828983824No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA633740.24888632656836204No Hit
CCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTAT523750.2056903675642687No Hit
CATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT493710.19389287135113142No Hit
CCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGA476270.18704372574670022No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT475830.18687092620163429No Hit
CCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTG467970.18378409796477482No Hit
CAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG432450.1698344619630892No Hit
GCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAG429000.16847955643927687No Hit
ATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCT420060.16496858386452828No Hit
TGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTA407840.160169469226561No Hit
TCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGT398640.15655638782972806No Hit
TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTA397690.15618329790288116No Hit
CGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGAT394300.15485195595339596No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG383910.15077153033240742No Hit
GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATAT373870.14682855889499405No Hit
CAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTC373450.14666361387470384No Hit
GTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCC363370.142704933387739No Hit
TCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGT359400.14114581021975783No Hit
ATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCA356360.1399519224538478No Hit
TTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGAC355010.13942174203148644No Hit
GATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTA347410.13643702261671137No Hit
TTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCT346200.13596182386778008No Hit
CTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGA340410.13368793894520803No Hit
GGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTT339860.1334719395138756No Hit
CCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGC330360.12974104024540678No Hit
ACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTG307650.12082222736257234No Hit
CATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGC305190.11985612081515828No Hit
ATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTC299820.11774718091287642No Hit
ATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAA297850.11697351022246763No Hit
TTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGAT296710.11652580231025138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286110.11236290417911772No Hit
TAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT283310.11126327071051638No Hit
CTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTGC279350.10970807480492308No Hit
AACCATATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCT274430.10777586171009498No Hit
GGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTAC270500.10623244759166525No Hit
GTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATA269200.10572190348124319No Hit
GTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTACTTCCCGTTT265610.10431201628400076No Hit
ATCAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTGGGTAC259630.10196351337605934No Hit
TATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCA256780.10084424359551869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1546100.092.428581
AGCAGTG1659750.085.923692
TGGTATC1895200.075.1456457
GCAGTGG1901850.074.845913
CAGTGGT1908200.074.6394654
GTGGTAT1923050.074.142726
GGTATCA1925350.073.988978
AGTGGTA1940850.073.4797745
GTATCAA1975400.072.1681759
AGAGTAC1933250.040.29374318-19
CAACGCA1944050.040.2585312-13
GCAGAGT1942250.040.20956416-17
ATCAACG1986600.039.48397410-11
TACATGG1983650.038.7010722-23
ACGCAGA2092800.037.46069714-15
AGTACAT2072450.037.40360620-21
CATGGGG631200.037.05942524-25
TCAACGC1947900.036.61345712-13
CAGAGTA1951450.036.48430318-19
AACGCAG1974250.036.17376314-15
Produced by FastQC (version 0.11.5)