FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_15hpf1SBC_202009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_15hpf1SBC_202009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24601752
Sequences flagged as poor quality0
Sequence length110
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGCTATTTCTCTGGGTACCTTAGCACCGTTCCTCGCTATTATTCCAA757901030.806789695303No Hit
TGCCGCTATTTCTCTGGGTACCGGACACCATTTCTCGCTATTATTCCAAC532260221.635052658038337No Hit
TGCCGCTATTTCTCTGGGTACCCGCAACAGTTTCTCGCTATTATTCCAAC412449816.765058033265273No Hit
TGCCGCTATTTCTCTGGGTACCTGAGATAAGTTCTCGCTATTATTCCAAC395202316.063990076804284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1550900.6304022575302767No Hit
TGCCGCTATTTCTCTGGGTACCAGTGGTCCTTTCTCGCTATTATTCCAAC1330570.540843595204114No Hit
TGCCGCTATTTCTCTGGGTACCAGCCTCTCATTCTCGCTATTATTCCAAC1283130.5215604156972236No Hit
TGCCGCTATTTCTCTGGGTACCATCACCTGGTTCTCGCTATTATTCCAAC879230.3573851163120415No Hit
TGCCGCTATTTCTCTGGGTACCACATGAGCATTCTCGCTATTATCCAACC861530.35019050675740493No Hit
TGCCGCTATTTCTCTGGGTACCTTAGCACCGTTCCTCGCTATTATTCAAA422380.17168695953036192No Hit
TGCCGCTATTTCTCTGGGTACCGGACACCATTTCTCGCTATTATTACAAC310530.12622271779668376No Hit
TGCCGCTATTTCTCTGGGTACCTTAGCACCGTTCCTCGCTATTATTACAA303060.12318634867955745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGTCC252.6973612E-7103.994615
TGCCCAT351.6189006E-10103.992721
TCCCGCG450.0103.992721
TACCCAT201.1132715E-5103.9927141
AGACGCT3650.0103.9927141
TTACGAT600.0103.9927141
CGTAGTT201.1133729E-5103.990814
GCCACTA14900.0103.98682
TGCCGCT23503600.0103.876571
GCCGCTA23559800.0103.78952
CCGCTAG16100.0103.666393
TGCCACT14300.0103.6291051
CCGCTAT23617450.0103.6067053
GACGCTA84200.0103.554542
CTATTTC23659150.0103.468186
GCTATTT23731300.0103.392295
TATTTCT23683200.0103.365137
TGACGAT8200.0103.358611
TTTCTCT23623250.0103.125299
TGCCCTA21350.0103.018551
Produced by FastQC (version 0.11.5)