FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf2_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf2_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13942232
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC251600.18045891073968645No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140110.10049323522948118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT745750.091.997691
AGCAGTG806700.084.853522
GCAGTGG869700.078.56593
AGTGGTA897900.076.432045
GTGGTAT909600.075.421686
GGTATCA920150.074.797218
CAGTGGT919450.074.50334
GTATCAA952550.072.307389
TGGTATC1154450.059.848697
CAACGCA920100.040.73990612-13
GCAGAGT922250.040.54046216-17
ACGCAGA944200.039.6788714-15
ATCAACG960300.039.13956510-11
TACATGG969900.038.0615222-23
CATGGGG373650.037.9265524-25
TCAACGC928450.037.13601312-13
AGAGTAC1012500.036.8726618-19
CGCAGAG936200.036.81735216-17
AACGCAG941800.036.58278714-15
CAGAGTA947750.036.30805218-19
Produced by FastQC (version 0.11.5)