FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf2_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf2_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18163333
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC331040.18225729826128276No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189910.10455680133156177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT948500.092.691411
AGCAGTG1038500.084.6096652
GCAGTGG1128650.077.701883
AGTGGTA1170450.075.043355
GTGGTAT1180200.074.372176
GGTATCA1194400.073.498918
CAGTGGT1197900.073.246714
GTATCAA1233400.071.094289
TGGTATC1483000.059.4215557
GCAGAGT1194250.040.30280716-17
CAACGCA1194400.040.2844112-13
ACGCAGA1225700.039.27983514-15
ATCAACG1239150.038.9231110-11
TACATGG1259050.037.7595922-23
CATGGGG485500.037.759424-25
AGAGTAC1314100.036.58901618-19
TCAACGC1200150.036.58597612-13
CGCAGAG1218150.036.1822216-17
AACGCAG1225350.035.90645614-15
CAGAGTA1226600.035.89859818-19
Produced by FastQC (version 0.11.5)