FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf2_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf2_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13558860
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC248890.18356262989661373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT721200.092.006781
AGCAGTG784050.084.489392
GCAGTGG847700.078.0700453
AGTGGTA876950.075.564015
GTGGTAT888100.074.622276
GGTATCA899950.073.7453168
CAGTGGT898700.073.680714
GTATCAA931150.071.210219
TGGTATC1115600.059.694047
CAACGCA898300.040.30390512-13
GCAGAGT897750.040.29881716-17
ACGCAGA918750.039.41551214-15
ATCAACG932800.038.90187510-11
TACATGG945550.037.7838322-23
CATGGGG358700.037.273824-25
TCAACGC904450.036.66049212-13
AGAGTAC984200.036.65778718-19
CGCAGAG911900.036.42012416-17
AACGCAG917050.036.19384814-15
CAGAGTA924500.035.9200818-19
Produced by FastQC (version 0.11.5)