FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf2_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf2_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14397098
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC265820.184634431188841No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144430.10031882814161576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT764400.092.4557341
AGCAGTG830600.084.882732
GCAGTGG904700.077.754083
AGTGGTA935850.075.274715
GTGGTAT944200.074.5722966
GGTATCA959050.073.504878
CAGTGGT958200.073.490014
GTATCAA989250.071.320959
TGGTATC1189250.059.5555277
CAACGCA955500.040.4028312-13
GCAGAGT953500.040.38583416-17
ACGCAGA979200.039.41949514-15
ATCAACG995500.038.8409510-11
TACATGG1010050.037.6855922-23
CATGGGG390900.037.1903824-25
TCAACGC964100.036.59565712-13
AGAGTAC1051050.036.5876918-19
CGCAGAG971900.036.29049316-17
AACGCAG976900.036.2145114-15
CAGAGTA979800.035.95240418-19
Produced by FastQC (version 0.11.5)