FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf1_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf1_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6904063
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC118450.17156564185465864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT75430.1092545070924179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT362550.092.256431
AGCAGTG391600.085.274872
GCAGTGG419050.079.5181053
AGTGGTA438050.075.979635
GTGGTAT441450.075.324326
CAGTGGT447750.074.3779144
GGTATCA448100.074.18388
GTATCAA463350.071.657129
TGGTATC549950.060.6517527
CAACGCA447800.040.4075412-13
GCAGAGT449700.040.14906716-17
ATCAACG462500.039.1284310-11
ACGCAGA465350.038.9181414-15
TACATGG469600.037.8800122-23
CATGGGG186000.037.3560524-25
TCAACGC450400.036.9933612-13
AGAGTAC494950.036.4205918-19
AACGCAG459800.036.32282614-15
CGCAGAG462200.036.17176416-17
CAGAGTA462750.036.06683318-19
Produced by FastQC (version 0.11.5)