FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf1_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf1_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8581510
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC148630.17319795700290508No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT93490.10894353091705307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT450350.092.326121
AGCAGTG486950.085.233282
GCAGTGG523850.079.05313
AGTGGTA542500.076.502365
GTGGTAT549000.075.522596
GGTATCA551450.075.210298
CAGTGGT558000.074.2828144
GTATCAA569700.072.768719
TGGTATC687300.060.5235257
CAACGCA555600.040.5725212-13
GCAGAGT557050.040.43216-17
ATCAACG571900.039.50592810-11
ACGCAGA575650.039.16818214-15
TACATGG583650.037.86445622-23
CATGGGG226250.037.45509324-25
TCAACGC557950.037.1952312-13
AGAGTAC613300.036.60021618-19
AACGCAG567500.036.56908814-15
CAGAGTA569500.036.40180618-19
CGCAGAG573200.036.27168716-17
Produced by FastQC (version 0.11.5)