FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf1_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf1_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7213303
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC123600.17135007360705629No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT80050.11097551288223995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT387450.091.736511
AGCAGTG420450.084.371492
GCAGTGG451300.078.547513
AGTGGTA468200.075.805325
GTGGTAT472600.075.089616
GGTATCA476550.074.446428
CAGTGGT479500.073.974484
GTATCAA493200.071.863859
TGGTATC579500.061.4989287
CAACGCA475850.040.7739912-13
GCAGAGT478750.040.4502716-17
ATCAACG491650.039.6374910-11
ACGCAGA490700.039.5984814-15
TACATGG500650.038.2581722-23
CATGGGG199300.038.04700524-25
AGAGTAC522500.037.08331718-19
TCAACGC478400.037.07819712-13
AACGCAG486900.036.505814-15
CGCAGAG487950.036.4371416-17
CAGAGTA487300.036.3151318-19
Produced by FastQC (version 0.11.5)