FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf1_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf1_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8274245
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC146470.17701917214198998No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT94300.11396810222564113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT436850.092.054571
AGCAGTG474700.084.546252
GCAGTGG505100.079.3464363
AGTGGTA524850.076.609235
GTGGTAT530450.075.8120966
GGTATCA532050.075.557088
CAGTGGT537000.074.797984
GTATCAA553150.072.7241449
TGGTATC656100.061.515947
CAACGCA536400.040.7817712-13
GCAGAGT537950.040.64357816-17
ATCAACG551950.039.72113410-11
ACGCAGA553400.039.65572414-15
CATGGGG221950.038.26268824-25
TACATGG564650.038.1386322-23
TCAACGC539700.037.28037312-13
AGAGTAC586000.037.19934518-19
AACGCAG548950.036.74680714-15
CGCAGAG551900.036.58697516-17
CAGAGTA549550.036.56297718-19
Produced by FastQC (version 0.11.5)