FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf1-1_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf1-1_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15588188
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237100.15210234826523775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT180900.11604940869330033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT700100.090.481271
AGCAGTG759650.083.294532
GCAGTGG811450.077.774773
AGTGGTA847350.074.555925
GTGGTAT853100.074.060926
GGTATCA859150.073.60378
CAGTGGT883300.071.5481954
GTATCAA896950.070.5159159
TGGTATC985000.064.349827
CAACGCA854600.040.67366412-13
GCAGAGT855200.040.51975616-17
ATCAACG894100.039.04165610-11
ACGCAGA894250.038.9314314-15
TACATGG900300.037.8642522-23
CATGGGG347250.037.6129124-25
TCAACGC863850.036.63813812-13
CAGAGTA880900.035.8479218-19
AACGCAG884700.035.81593314-15
CGCAGAG883900.035.8093616-17
AGAGTAC1004700.034.46637718-19
Produced by FastQC (version 0.11.5)