FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf1-1_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf1-1_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18351775
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281350.15330942102330702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT209930.11439220456876788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT821750.090.980921
AGCAGTG894350.083.4980852
GCAGTGG964800.077.3013843
AGTGGTA999200.074.669155
GTGGTAT1010050.073.889056
GGTATCA1013600.073.723818
CAGTGGT1046300.071.345294
GTATCAA1061900.070.392229
TGGTATC1160550.064.530457
CAACGCA1008150.040.8996112-13
GCAGAGT1009750.040.69569816-17
ATCAACG1054850.039.16452810-11
ACGCAGA1056300.039.0428514-15
TACATGG1061600.038.0358122-23
CATGGGG406850.037.20960624-25
TCAACGC1022800.036.5767912-13
AACGCAG1040800.035.9893814-15
CAGAGTA1040900.035.87998218-19
CGCAGAG1045400.035.7835216-17
GAGTACA1072650.034.67359520-21
Produced by FastQC (version 0.11.5)