FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf1-1_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf1-1_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20135087
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300550.1492667997908328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT231340.11489396594114544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT933450.091.32311
AGCAGTG1007100.084.516262
GCAGTGG1085600.078.2351763
AGTGGTA1124150.075.690365
GTGGTAT1140800.074.5508656
GGTATCA1143250.074.4742668
CAGTGGT1179100.072.1376654
GTATCAA1195450.071.2863859
TGGTATC1312000.065.048467
CAACGCA1145600.040.773512-13
GCAGAGT1142100.040.75886516-17
ATCAACG1193600.039.20879410-11
ACGCAGA1194700.039.13314414-15
TACATGG1198300.038.18830522-23
CATGGGG458500.037.5717624-25
TCAACGC1158450.036.8129812-13
AACGCAG1181000.036.16120514-15
CAGAGTA1179550.036.12261218-19
CGCAGAG1181750.036.0887316-17
GAGTACA1218900.034.79498720-21
Produced by FastQC (version 0.11.5)