FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n02_CRISPR_12hpf1-1_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n02_CRISPR_12hpf1-1_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15862649
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236700.1492184565137891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT180310.11366953905365994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT730750.091.433831
AGCAGTG785200.084.8225562
GCAGTGG843850.078.781753
AGTGGTA880600.075.575015
GTGGTAT887400.074.991226
GGTATCA889050.074.8936848
CAGTGGT918800.072.436064
GTATCAA931150.071.544419
TGGTATC1019650.065.543177
CAACGCA886950.041.17514412-13
GCAGAGT888150.041.0485316-17
ATCAACG922800.039.6931810-11
ACGCAGA930000.039.3364214-15
TACATGG935050.038.33994722-23
CATGGGG355200.038.23558824-25
TCAACGC895750.037.24075312-13
AACGCAG917300.036.40004714-15
CAGAGTA915550.036.38577318-19
CGCAGAG921050.036.19458816-17
GAGTACA947600.035.06529220-21
Produced by FastQC (version 0.11.5)