FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32263813
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAG2037100.6313884846778649No Hit
TAACAATGGTCTTGTGGAAAGGACGAAA2011770.6235375837319662No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAA1798490.5574325638448252No Hit
ATACTGTATCTCTTGTGGAAAGGACGAA1753940.5436245244788642No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGA1514340.46936175832658095No Hit
GATCGATAACGCATTTCTTGTGGAAAGG1403640.43505087262934483No Hit
GATAGGTCGCATCTTGTGGAAAGGACGA904510.28034814112020795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGG586340.1817330146315936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG213100.020.6871761
GATACAG246100.020.5067566
CAATGGT242200.020.4600284
AATGGTC242800.020.3687065
CGATAGG216100.020.3001776
TAACGCA172500.020.1392367
CGATACA251250.020.0776045
TCGATAG219000.020.061435
GGTATTC251650.020.03629712
AACAATG249050.019.9777572
GATAACG173750.019.9503545
TAAGGTC222300.019.94595313
ACAATGG248450.019.9056553
CTGTATC208200.019.8766294
ATACAGG256300.019.7074457
CCCTTCC199100.019.6852159
ATCGATT186500.019.6388681
GATTCCT186350.019.6282714
CGATTCC186350.019.6225223
GTAAGGT226500.019.59557512
Produced by FastQC (version 0.11.5)