FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf2_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf2_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21045354
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA97200.020.214171
AAAGAAC183300.019.6377431
AGAACGT143650.019.5190433
GGGGTGA55850.019.4790345
GAAAAGA50750.019.3557384
ATGGACA86950.019.292773
GTGCACG86900.019.141361
GTGCTGG76800.019.1375371
CGAGGAA53750.019.1370031
TAGCCGA74050.019.1031997
CCCGAAC64800.019.0643422
AGACGCA65100.019.042818
GAACGTG96150.019.0026724
AAAAGAA42500.018.997725
CATGGAC80950.018.9707982
CGTGGCT95350.018.9081237
ACCGTGA76600.018.8992312
ACCTACC86650.018.8664861
CAAAGCT95400.018.8058838
TTGGGCG77450.018.7922691
Produced by FastQC (version 0.11.5)