FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf2_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf2_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31152914
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA141500.020.0041941
AAAGAAC265000.019.5903321
GGGGTGA87700.019.5547145
AGAACGT210950.019.523633
TAGCCGA105850.019.308327
AAAAGAA60450.019.2346215
AGACGCA95750.019.1965458
GTGCACG127550.019.1819171
GAACGTG143700.019.1452564
GTGCTGG119650.019.1336171
GAAAAGA73200.019.130294
CGTGGCT141350.019.0893577
ATGGACA130700.019.080083
ACCGTGA113050.019.0629042
CATGGAC119000.019.0064642
CCCGAAC91150.018.9844152
GACCGTG131050.018.9718251
CAAAGCT140500.018.9463048
AATGAAG102850.018.857571
TTGGGCG119900.018.8459911
Produced by FastQC (version 0.11.5)