FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf2_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf2_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26293911
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA114600.020.1015131
AAAGAAC222950.019.5005171
AGAACGT175950.019.4873163
GGGGTGA70550.019.3811455
AGACGCA81600.019.3574128
GAAAAGA62650.019.226384
TAGCCGA89800.019.2192027
CGTGGCT119600.019.1670517
GAACGTG115550.019.0970134
CAAAGCT120350.019.0656328
CATGGAC98300.019.0360832
AGGACGC73600.019.0104458
ACCTACC113250.018.9617421
GTGCTGG103400.018.927451
ATGGACA107850.018.920313
AAAAGAA51950.018.887945
GTGCACG105750.018.8605381
ACCGTGA96000.018.838982
GGAGAAC67850.018.8243081
CGATGCG90100.018.8154471
Produced by FastQC (version 0.11.5)