FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf2_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf2_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26186551
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA117350.020.1277371
AAAGAAC227350.019.6993621
AGAACGT179250.019.545563
GAAAAGA62350.019.5126634
TAGCCGA90150.019.4863477
GGGGTGA73550.019.3381335
GAACGTG121100.019.2388764
AGACGCA81800.019.1351958
CATGGAC98250.019.1240442
CCCGAAC77600.019.0954692
GTGCTGG97600.019.0832841
ATGGACA109700.019.0521873
CGTGGCT118900.018.9839577
GTGCACG103100.018.9295771
ACCGTGA97600.018.9132672
TTGGGCG95950.018.8954931
AAAGAGT80250.018.8613956
GACCGTG111650.018.8594571
AAAAGAA52800.018.812765
CAAAGCT119050.018.802538
Produced by FastQC (version 0.11.5)