FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf2SBC_202009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf2SBC_202009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28895933
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCG194700.020.5673711
TGACAGT303100.020.5434721
GATGCGG179900.020.4729822
GGGGTAG135750.020.347475
GGGGTGA117600.020.288775
CGATAGC206550.020.2483184
CTGAAGT189750.020.2148913
CGGGGTG108400.020.2144554
CATGGAC141050.020.2078952
GGTGGCG123700.020.2037687
GACAGGA129300.020.162956
AGCTCGC207550.020.1552548
GCTGAAG187650.020.155062
GCGGTTG182350.020.15465
GCTCGCA207450.020.1332289
TAGCTCG207900.020.0847077
CGAGGAA117150.020.0398961
AAGACGA116400.020.0340679
GGAAGAA97150.020.018239
TGCTGAA192950.020.01271
Produced by FastQC (version 0.11.5)