FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf1_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf1_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24682029
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCG104500.019.2967931
GAGAGGA67150.019.035485
GCCGAGA60500.019.0195142
GACTGGG108500.019.0190329
GGCGTAA96250.018.97114410
GGAACCC84700.018.872141
TAAGTCG82300.018.8209611
CCGAGAG61350.018.8089563
AAGCGGT85650.018.7897383
CGCAGGA61300.018.77132
ATGGCCG71900.018.58803710
CGGAGTA66400.018.5704679
ATGCAAA102250.018.48187410
GAGGAGC91350.018.4000324
GAAGAAT70050.018.3745481
TGGCGTA99750.018.3716169
CACTGGG101900.018.353281
AGCGGTG44300.018.3503554
CGCTGAG71250.018.2952043
CGCAGAA44750.018.288776
Produced by FastQC (version 0.11.5)