FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf1_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf1_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31494530
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCG139450.019.3831371
TAAGTCG111050.019.2383611
GCCGAGA78550.019.18662
GACTGGG134200.019.147149
GGAACCC105900.018.9896811
GAGAGGA86650.018.9155035
GAGGAGC114150.018.7913884
CCGAGAG80200.018.790853
GGCGTAA130600.018.79066310
AAGCGGT114100.018.7321383
CGCAGGA78050.018.618882
ATGGCCG96600.018.59493810
CGCTGAG97900.018.4160483
GAGGCTG109950.018.3779729
ATGCAAA131800.018.37754810
TGGCGTA133800.018.3658359
CGGAGTA79000.018.3515179
TCGAGGA70400.018.3450572
TCGGAGT83600.018.2758778
CACAGAT145900.018.2623351
Produced by FastQC (version 0.11.5)