FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf1_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf1_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26714018
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCG117900.019.6138361
GCCGAGA65000.019.1416382
AAGCGGT96250.019.1319543
GGCGTAA104250.018.92902610
GACTGGG116600.018.8485879
GAGAGGA73850.018.768295
GGAACCC89250.018.7484171
GAGGAGC99100.018.7371434
TAAGTCG94350.018.6444761
CGCAGGA66700.018.5053312
AAAAGGG54000.018.4560625
CCGAGAG67600.018.4368973
CGCTGAG82450.018.3983233
ATGGCCG80350.018.38535310
ATGCAAA116900.018.3297410
CGGAGTA68650.018.3141829
AAAGTGA75750.018.31371
GAGGCTG95800.018.2218849
TGGCGTA108800.018.1879679
TCGGCCA87200.018.15207110
Produced by FastQC (version 0.11.5)