FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf1_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf1_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25463030
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCG110650.019.2281381
GCCGAGA61900.019.1576942
TAAGTCG90600.019.0149731
GACTGGG106800.018.8687029
GGAACCC88850.018.8075621
GAGGAGC93500.018.7883574
CCGAGAG63350.018.7183533
CGCAGGA57750.018.7057272
AAGCGGT91200.018.6349893
GAGAGGA69750.018.5937425
GGCGTAA101450.018.4217510
CGGAGTA68300.018.4082859
GAGGCTG87900.018.370679
TCGGAGT72500.018.343248
AACGGGA57450.018.3254241
CGCTGAG78200.018.2724953
CGCAGAA44400.018.2344956
ATGGCCG80300.018.19164810
TCGGCCA83350.018.1858310
ATGCAAA104250.018.16965310
Produced by FastQC (version 0.11.5)