FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_15hpf1SBC_202009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_15hpf1SBC_202009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24601752
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG209150.020.8345261
GAAGCGG147900.020.5140842
TCGAGAG154850.020.4795638
TATGCAA239550.020.4384829
GGGCAGA111500.020.402245
CACAGAT265400.020.3607651
CGTTGGG173700.020.3555451
AAGCGGT159350.020.3505783
GAAGAAT174300.020.3485891
GTATGCA241000.020.3155128
GGACTGG162900.020.2845829
GAGAGGC156900.020.2751627
AGTATGC290850.020.2714447
GAGGAGC173350.020.268074
AAGCGAT173050.020.2389839
ACATGCA245700.020.2246781
ATGCAAA245650.020.21761710
ACGCACG112500.020.2127131
ACAGCCG159650.020.1623941
TCGAGGA119850.020.1567422
Produced by FastQC (version 0.11.5)