FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf2_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf2_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13942232
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGTTGGCATCGTTTATAGTCAGAAC178410.12796372919343185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACACC141500.020.7096567
AGGAACG72250.020.5530828
ATTATCC150200.020.3764741
TGCGGAG54400.020.2210623
GGTACAC115400.020.1989144
AGCAGGG85550.020.1864878
GAGACCC50350.020.1234281
GCAGAGG70700.020.0867524
AAGGAAC39650.020.0581177
AAAAGTC56200.020.0431755
GCAGTAC75400.019.9570989
GAGCTCC64750.019.91010710
CAGGGAG87000.019.87554610
CACACAA85450.019.8653261
ACAGAAA102350.019.8635081
TGAAGAC43450.019.8485685
ACACTAG75550.019.8456023
AAACGAA51450.019.8428671
AGCGAAA55500.019.819298
CTGAGCG50700.019.810921
Produced by FastQC (version 0.11.5)