FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf2_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf2_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18163333
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGTTGGCATCGTTTATAGTCAGAAC230320.126804920660762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACACC183500.020.543317
AGCAGGG119050.020.4565988
AGGAACG95900.020.4052778
ATTATCC194800.020.3868471
GCAGAGG93450.020.3288944
AAAAGTC75150.020.1267875
GCGAAAC72600.020.1209189
TGAGGGA77500.020.0856821
AAACGAA69850.020.0490511
AGGCTAT148250.020.0482759
GGTACAC147600.019.9807224
GCACGGA82600.019.9757737
CACACAA108200.019.9685271
CAGGGAG122300.019.93102610
GAGACCC67350.019.9112221
ACAGAAA132750.019.8970851
CAGGCTA150000.019.8656568
GCTGAAT125000.019.8457871
AGAGGAG74050.019.831563
CATAAAG120200.019.7765713
Produced by FastQC (version 0.11.5)