FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf2_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf2_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13558860
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGTTGGCATCGTTTATAGTCAGAAC175510.12944303577144392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAACG71800.020.7740028
ACACACC138900.020.5982847
ATTATCC153450.020.4116231
GCAGAGG66950.020.3245074
GGTACAC110800.020.2530924
AGCAGGG86550.020.2076118
GGCTCCC58100.020.1252849
TGAGGGA57450.020.0882661
GCTGAAT97650.020.087971
AGACCCA50350.020.0790982
AGGCTAT111700.020.0005369
AAACGAA51350.019.9679031
GAGACCC51850.019.966311
GCACGGA58850.019.9626277
ACAGAAA98900.019.9564421
GAGGATG58550.019.9518189
GCGAAAC57650.019.938939
GGCTATT112200.019.93101510
GACGACC40400.019.93033610
TGAGGAG43900.019.9232231
Produced by FastQC (version 0.11.5)