FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf2_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf2_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14397098
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGTTGGCATCGTTTATAGTCAGAAC182880.12702559918672499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACACC153750.020.540257
AGGAACG77700.020.3998818
ATTATCC153000.020.3841021
AGCAGGG90350.020.368198
GCAGAGG71850.020.1939284
GGTACAC124400.020.1789264
AGGCTAT114850.020.1320469
GCACGGA66000.020.0997687
GCGAAAC54400.020.0787439
AAACGAA53450.020.0672021
TGCGGAG54200.019.9709433
ACAGAAA104450.019.969161
TGAAGAC43600.019.9568845
GAGACCC53950.019.9220031
CAGGGAG92550.019.8840210
CAGGCTA116700.019.8693838
GCTGAAT103400.019.8634051
TGAGGAG49900.019.841381
TACACAC94550.019.8366626
AGCGAAA55150.019.7856718
Produced by FastQC (version 0.11.5)