FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf1_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf1_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6904063
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCCC332300.021.5040825
CATCCCT332450.021.4945376
TCATTCA336650.021.4758011
TTCATCC333950.021.4670094
CCTTCCC333600.021.4554710
CCCTTCC335450.021.4256789
ATCCCTT334500.021.4245157
ATTCATC335250.021.416583
CATTCAT336500.021.3505652
GGAGCAG82450.021.3335464
CGGGAGG42850.021.0764564
CATCGGG87050.021.0540981
TCGGGTG73800.021.0031811
GAGGAAG73150.020.9930883
GGCGTAG42150.020.9828133
TCCCTTC342850.020.9760658
AGGAGCA57750.020.9720363
AGCCGAG39350.020.9383453
AGGAGAC34200.020.90597710
ATGGGAG47250.020.8843541
Produced by FastQC (version 0.11.5)