FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf1_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf1_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8581510
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTCA420350.021.5642971
CATCCCT417250.021.5421946
ATCCCTT418350.021.5189847
TCATCCC417950.021.5112555
TTCATCC418900.021.480854
CCTTCCC419600.021.4673610
CCCTTCC421550.021.43599
ATTCATC420450.021.4095123
CATTCAT421550.021.4047222
GGAGCAG103300.021.3405694
ATGGGAG59250.021.1127091
CGGGAGG54100.021.1061334
TCCCTTC430750.020.975158
AGGAGCA72700.020.971883
GAGGAAG91700.020.9331533
GGTAATC110150.020.9252851
GGAAAGA54050.020.9209149
TACTGAC82200.020.9156049
CATCGGG109000.020.9139021
TAGGAGC72950.020.9014722
Produced by FastQC (version 0.11.5)